What is the Enzyme Activity Calculator?

The Enzyme Activity Calculator estimates the activity of an enzyme based on experimental data, including changes in absorbance, reaction time, extinction coefficient, path length, and sample volume. By using standard biochemical formulas, it provides the enzyme activity in µmol/min, helping students, researchers, and lab professionals quickly analyze enzyme kinetics, monitor reactions, and validate experimental results efficiently.

How to Use:

1. Enter Change in Absorbance (ΔA): Input the observed change in absorbance during the reaction.
2. Enter Time: Type the duration of the reaction in minutes.
3. Enter Extinction Coefficient (ε): Input the extinction coefficient in L/mol·cm.
4. Enter Path Length: Provide the path length of the cuvette in cm.
5. Enter Volume of Sample: Input the sample volume in milliliters (mL).
6. Click “Calculate”: The calculator will display the enzyme activity in µmol/min.
7. Review the Result: Use this data to analyze enzyme kinetics, compare samples, or plan further experiments.